Research Summary - Tissue culture models

Our group’s research interests are focused on the generation and analysis of cell culture models for brain disorders.

The key areas of interest where we have developed expertise are:

  1. Analysis of cell lines

  2. Generation and analysis of primary neurons from mice and rats

  3. Generation and analysis of human induced pluripotent stem cells

We have developed expertise in the generation and analysis of cells: Generation of stem cells from human hair from patients or mutated using CRIPSR, and differentiation into Neurons and Enterocytes

Viability assays, Analysis of cells (setup, function, composition), Analysis of Biometals

Current projects:

Characterization of cells from patients with Acrodermatitis enteropathica.

Characterization of zinc absorption in enterocytes

Characterization of the role of zinc transporters in neurons

RESEARCH experience:


Pfaender S, Sauer AK, Hagmeyer S, Mangus K, Linta L, Liebau S, Bockmann J, Huguet G, Bourgeron T, Boeckers TM, Grabrucker AM. Zinc deficiency and low enterocyte zinc transporter expression in human patients with autism related mutations in SHANK3, SciRep, 2017; 7:45190.

PFAENDER S, FÖHR K, LUTZ AK, PUTZ S, ACHBERGER K, LINTA L, LIEBAU S, BOECKERS TM, GRABRUCKER AM: Cellular zinc homeostasis contributes to neuronal differentiation in human induced pluripotent stem cells. Neural Plasticity 2016; 2016: 3760702.

Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM: Effects of trace metal profiles characteristic for autism on synapses in cultured neurons. Neural Plasticity 2015; 2015: 985083

Grabrucker S, Proepper C, Mangus K, Eckert M, Chhabra R, Schmeisser MJ, Boeckers TM, Grabrucker AM. The PSD protein ProSAP2/Shank3 displays synapto-nuclear shuttling which is deregulated in a schizophrenia-associated mutation. Exp Neurol 2014; 253: 126-37.

Schmeisser MJ, Kühl S, Beth N, Weis TM, Schoen M, Grabrucker AM, Kühl M, Boeckers TM. The Nedd4 binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons. Neural Development 2013, 8 (1): 18.

Arons M, Thynne CJ, Grabrucker AM, Li D, Schoen M, Cheyne JE, Boeckers TM, Montgomery JM, Garner CC. Autism associated mutations in ProSAP2/Shank3 impair synaptic transmission and neurexin-neuroligin mediated transsynaptic signaling. J. Neurosci. 2012, 32 (43): 14966-78.

Grabrucker AM, Knight MJ, Proepper C, Bockmann J, Joubert M, Rowan M, Nienhaus GU, Garner CC, Bowie JU, Kreutz MR, Gundelfinger ED, Boeckers TM: Concerted action of zinc and ProSAP/Shank in synaptogenesis and synapse maturation. EMBO Journal 2011, 30 (3): 569-581.

GRABRUCKER AM, VAIDA B, BOCKMANN J, BOECKERS TM: Synaptogenesis of hippocampal neurons in primary cell culture, Cell and Tissue Research. 2009, 338: 333–341.

SCHMEISSER MJ, GRABRUCKER AM, BOCKMANN J, BOECKERS TM: Synaptic crosstalk between NMDA receptors and LAPSER1/ß-catenin at excitatory synapses. J Biol Chem. 2009, 284(42): 29146-29157.

GRABRUCKER AM, VAIDA B, BOCKMANN J, BOECKERS TM: Efficient targeting of proteins to postsynaptic densities of excitatory synapses using a novel pSDTarget vector system. Journal of Neuroscience Methods. 2009, 181(2): 227-234.